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Author:Yih-Juh Shiau, Chi-Ni Hsia* and Hsin-Sheng Tsay
Abstract:
Haemaria discolor (Ker) Lindl. is cultivated for the purpose of ornamental and medicinal in Taiwan. We have optimized a method for artificial self-pollination of H. discolor and for proloning capsules storage life by using cold treatment in this study. The highest fruit set (100%) and embryo germination (44.6-45.6%) were obtained when the pollinia and ovules from flower stage of 0-5 DAF (days after flowering) used for pollination. The germination rate of massules from 3-5 DAF stage were 98.4% (82.6 pollen tubes/massule) when culturing in the liquid medium containing 10% sucrose with 0.01% boric acid for l8hrs. Cold treatment on capsules at 5±1℃ with 100% relative humidity was found effective to prolong seed longevity to 28 days.
Key words:Haemaria discolor, Orchidaceae, Pollination, Seed germination
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