Structural Elucidation of Three Novel Kaempferol O-tri-Glycosides that Are Involved in the Defense Response of Hybrid Ornithogalum to Pectobacterium carotovorum
1
Department of Ornamental Horticulture, ARO, Volcani Center, Derech Hamacabim 20 P.O. Box 6, 50250 Bet-Dagan, Israel
2
Institute of Biochemistry, Food Science and Nutrition, The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, 7610001 Rehovot, Israel
3
Department of Chemistry, Bar Ilan University, 52900 Ramat-Gan, Israel
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(16), 2910; https://doi.org/10.3390/molecules24162910
Submission received: 9 July 2019
/
Revised: 5 August 2019
/
Accepted: 9 August 2019
/
Published: 10 August 2019
(This article belongs to the Special Issue Natural Products in Plant Protection)
Abstract
:Ornithogalum is an ornamental flowering species that grows from a bulb and is highly susceptible to soft-rot disease caused by Pectobacterium carotovorum (Pc). Interspecific hybridization between O. thyrsoides and O. dubium yielded hybrids with enhanced resistance to that pathogen. The hybrids displayed distinct phenolic-compound profiles with several peaks that were specifically heightened following Pc infection. Three of these compounds were isolated and identified as novel kaempferol O-tri-glycosides. The structures of these compounds were elucidated using reversed phase high-performance liquid chromatography (RP-LC), RP-LC coupled to high-resolution mass spectrometry (RP-LC-MS), and nuclear magnetic resonance (NMR) (1D 1H and 13C, DEPT, HMQC, HMBC, COSY, and NOE), in order to achieve pure and defined compounds data. The new compounds were finally identified as kaempferol 3-O-[4-O-α-l-(3-O-acetic)-rhamnopyranosyl-6-O-β-d-xylopyranosyl]-β-d-glucopyranoside, kaempferol 3-O-[4-O-α-l-(2-O-acetic)-rhamnopyranosyl-6-O-β-d-xylopyranosyl]-β-d-glucopyranoside and kaempferol 3-O-[4-O-α-l-(2,3-O-diacetic)-rhamnopyranosyl-6-O-β-d-xylopyranosyl]-β-d-glucopyranoside.
1. Introduction
The genus Ornithogalum, also known as the “Star of Beth-Lehem”, belongs to the family Asparagaceae, which includes about 250–300 species [1]. These include wild and cultivated species that are widely distributed across Europe, Asia (reaching as far east as Afghanistan), Africa and Madagascar [2,3,4]. Most members of this genus are herbaceous perennials, spring- and summer-flowering bulb plants. In recent decades, the African varieties of the plant (along with some others) have been grown commercially and sold as cut flowers and potted plants in South Africa, the USA, the Netherlands and Israel. The plant’s potential as a cut flower and garden plant is severely hampered by its susceptibility to bacterial soft rot caused by Pectobacterium carotovorum (Pc) species. Several attempts have been made to minimize soft-rot disease through the use of plant activators that induce systemic resistance and there have also been efforts to develop resistant clones through molecular and classical breeding [5,6,7]. Such strategies have involved the induction and accumulation of secondary metabolites, resulting in reduced bacterial pressure and multiplication, or direct interference with bacterial virulence [8]. In this context, external application of methyl jasmonate (MJ) has been shown to activate the jasmonic-acid signaling pathway, which plays a central role in the regulation of secondary-metabolite biosynthesis in tomato (Solanum lycopersicum) [9,10,11,12]. In O. dubium and Zantedeschia aethiopica (another ornamental monocot), defense elicitation with exogenous MJ has been shown to reduce disease symptoms and lead to increased accumulation of polyphenolic compounds following Pc infection [6,13].
Plant phenolics are considered to be the most abundant secondary metabolites isolated from plants. To date, over 8000 phenolic structures (with simple molecular structures or polymeric structures) have been discovered [14,15]. Most polyphenols appear in nature as glycosides with one or more glycosidic moieties. They are involved in essential processes, such as growth and reproduction, and many help protect plants from biotic and abiotic stress [14,15,16,17]. Flavonoids are known to play important roles in plant tissues, including providing protection against UV-B radiation, as antioxidants, as antifeedants and as phytoalexins [18,19,20,21,22]. Some flavonoids are synthesized in response to plant pathogens [15,16,21,23].
Here, two Ornithogalum species, O. dubium, which is highly susceptible to soft rot, and O. thyrosoides, which is relatively resistant, were crossed to yield interspecific F1 hybrids with different levels of resistance to the soft-rot pathogen. In correlation with their observed resistance to soft rot, these hybrids produced metabolites in response to infection with Pc, and those metabolites exhibited patterns of UV absorption that are typical of flavonoids. Three flavonoids were further purified from leaf extracts and mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy were used to identify them as three novel kaempferol O-tri-glycosides.
2. Results and Discussion
Interspecific breeding was carried out between two Ornithogalum accessions: O. dubium (#49_60) and O. thyrosoides (#36_1) [24]. Following embryo rescue, the cross yielded two F1 hybrids, designated #2_28 and #2_32, and micropropagation protocols were used to clone those hybrids (Figure 1D) for further analysis [25]. Infiltration with Pc showed that both hybrids are less sensitive to soft-rot infection than the parent #49_60, with #2_28 being the more resistant (Figure 1A). Following infiltration with Pc, leaves were extracted with aqueous methanol, as described previously [13], and the levels of phenolic compounds in the extracts were determined, revealing an inverse correlation between sensitivity to Pc and levels of phenolics (expressed as catechin equivalents, Figure 1B). All extracts were then separated and characterized using reversed phase high performance liquid chromatography (RP-LC) and photo diode array detection, and a unique profile of phytochemicals was observed for each plant line (Figure 1C). Each of the plant lines had a typical color phenotype: The pollen donor was white, the female flower orange and the F1 hybrids were light orange (Figure 1D). Pc infection was found to induce the production of several compounds as a part of the general plant response to the bacterium with more than two-fold increases in the compounds assigned Peak Numbers 1, 2 and 3. Hyphenated and complementary spectral analyses were used to further characterize these three molecules.
2.1. Mass Spectrometry of Compound 1
Using ESI-MS (electrospray ionization mass spectrometry) in the negative mode for Compound 1 revealed a molecular ion [M − H]− at m/z 767, indicating a molecular formula of C34H39O20, which was confirmed by the observation of the positive mode [M + Na]+ ion at m/z 791 (Figure 2A). Significant fragment ion peaks, identified by MS2 revealed the presence of an ion at m/z 725 [767 (M − H) − 42 (CH3CO)]−, due to a loss of an acetyl moiety, and at 707 [767 (M − H) − 60 (CH3CO and water)]−, due to additional water removal.
Extensive MS2 and MS3 fragmentations (Figure 2B), showed the relevant peaks of other important fragments: m/z 561 [767 (M − H) − 206 (− acetyl rhamnose (Rha) − H2O)]−, m/z 393 [767 (M − H) − 374 (− acetyl Rha, xylose (Xyl) and 3H2O)]− and m/z 285 (m/z kaempferol aglycone)].
2.2. NMR Analyses of Compound 1
Additional cleanup of the compound was performed using solid-phase extraction (SPE) through a single-use sep-pak™ C-18, prior to NMR analyses. Indeed, the NMR spectra supported the identification of Compound 1 as kaempferol-O-tri-glycoside (1, Figure 3): The 1H-NMR spectrum of Compound 1 showed four types of aromatic protons [δ 8.08 (2H, AA′XX′ system), 6.90 (2H, AA′XX′ system), 6.39 (1H, d, J = 2 Hz) and 6.18 (1H, d, J = 2 Hz)] representing the kaempferol aglycone, together with three anomeric protons [δ 5.65 (1H, d, J = 7.5 Hz, HGlc-1), 5.20 (1H, d, J = 2 Hz, HRha-1) and 4.07 (1H, d, J = 7.5 Hz, HXyl-1)]. The COSY analysis and anomeric coupling constant values (JGlc,Xyl = 7.5 Hz, JRha = 2 Hz) allowed complete identification of the spin systems of β-d glucose (Glc), β-d Xyl and α-l Rha. In addition, 34 carbon signals were observed in the 13C-NMR spectrum (Table 1 and Table 2 and Figure 3). Among them, 15 carbons were assigned to the kaempferol unit and three anomeric carbon signals [δ 105.14, 102.70 and 100.26] to the sugar moiety. For the glucose moiety, CGlc-4 and CGlc-6 were deshielded (δ 71.83, 69.46) due to glycosylation at these two positions. An acetate moiety was also observed.
The connectivity of the kaempferol, the three sugar moieties, and the acetate unit was deduced using HMBC correlations (Figure 4). Glc in this molecule was linked to the hydroxyl at C-3 of kaempferol with a 3JC-H correlation between the HGlc-1 and C-3 of the aglycone. It was also deduced from an HMBC cross-peak, that the anomeric protons of Xyl and Rha are correlated with CGlc-6 and CGlc-4, respectively. Accounting for all the spectroscopic data described above, the structure of 1 was confirmed as kaempferol 3-O-[4-O-α-l-(3-O-acetic)-rhamnopyranosyl-6-O-β-d-xylopyranosyl]-β-d-glucopyranoside.
2.3. Spectral Analysis of Compound 2
Comparison of the mass spectra (ESI-MS) of Compounds 2 and 1 revealed the presence of similar molecular ions in both the positive and the negative modes ([M + Na]+ at m/z 791 and [M − H]− at m/z 767), resulting in the molecular formula C34H39O20. The MS2 fragmentation of those molecular peaks was similar, indicating that Flavonoids 1 and 2 are isomers. Data acquired using NMR analyses similar to those described above allowed the identification of Compound 2 as a kaempferol aglycone bound to three monosaccharide units. The 1H-NMR spectrum showed four types of aromatic protons [δ 6.89 (2H, AA’XX’ system), 8.07 (2H, AA’XX’ system), 6.39 (1H, d, J = 2 Hz), and 6.17 (1H, d, J = 2 Hz)] due to kaempferol, along with three anomeric protons [δ 5.64 (1H, d, J = 7.5 Hz, HGlc-1)], 5.21 (1H, d, J = 2 Hz, HRha-1) and 4.06 (1H, d, J = 7.5 Hz, HXyl-1)] (Table 1 and Table 2, Figure 3).
The 1H-NMR spectra of Compounds 1 and 2 were almost identical except for signals arising from the rhamnose acetate moiety. In the NMR analysis, it was concluded that Compound 2 is an isomer of Compound 1 in which the acetate group is attached to position 2 rather than position 3 of the rhamnose moiety. To conclude, compound 2 was identified as kaempferol 3-O-[4-O-α-L-(2-O-acetic)-rhamnopyranosyl-6-O-β-d-xylopyranosyl]-β-d-glucopyranoside.
2.4. Spectral Analysis of Compound 3
The spectral data acquired for Compound 3 resembled the spectral data for Compounds 1 and 2. Compound 3 had a molecular ion peak [M − H]− at m/z 809 in the negative ESI mode, which indicates a molecular formula of C36H41O21. As above, this was confirmed by the ion [M + H]+ at m/z 811 in the positive mode. Fragment ion peaks of MS2 and MS3 at m/z: 767 [809 (M − H)-42 (CH3CO)]−, 561 [809 (M − H) − 248 (-diacetylated rhamnose and H2O)]−, 393 [809 (M − H) − 416 (-diacetylated rhamnose, xylose and 3H2O)]− and 285 [809 (M − H) − 524 (kaempferol aglycone)]. Compound 3 was shown to have a kaempferol aglycone with four aromatic protons [δ 6.91 (2H, AA’XX’ system), 8.07 (2H, AA’XX’ system), 6.40 (1H, d, J = 2 Hz), and 6.19 (1H, d, J = 2 Hz)], and three anomeric protons [δ 5.64 (1H, d, J = 7.5 Hz, HGlc-1)], 5.20 (1H, d, J = 2 Hz, HRha-1) and 4.07 (1H, d, J = 7.5 Hz, HXyl-1)] and three anomeric carbons [100.25, 105.15, 100.07]. Two acetylated positions on Rha were deuced from the two 1H-NMR singlets at δ 2.01 and 2.09 (CRha-2 and CRha-3), and confirmed by 13C-NMR signals at δ 20.94 and 20.75 (methyls) and δ 171.79 and 172.41 (carbonyls; CRha-2 and CRha-3, respectively; Table 1 and Table 2). These NMR spectra, supported by the results of COSY, DEPT, HMQC, HMBC, and NOE experiments, allowed us to identify Compound 3 as kaempferol 3-O-[4-O-α-l-(2,3-O-diacetic)–rhamnopyranosyl-6-O-β−xylopyranosyl]-β-d-glucopyranoside.
3. Experimental
3.1. General
All solutions were prepared in DDW (double distilled water), unless indicated otherwise. All materials were purchased, from Sigma-Aldrich, unless otherwise indicated. HPLC grade methanol, ethanol, acetonitrile, ethyl acetate, hexane were purchased from Baker (Phillipsburg, NG, USA). All tissue culture materials were purchased from Duchefa (Haarlem, The Netherlands).
RP-LC-MS analyses were performed using Accela High-Speed LC system coupled with linear trap quadrupole (LTQ) Orbitrap Discovery hybrid mass spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) equipped with electrospray ionization source. The mass spectrometer was operated in both negative and positive ionization modes, and ion source was set as follows: Spray voltage 3 kV, capillary temperature 250 °C, ion-transfer optics parameters were optimized using automatic tune option, sheath gas rate (arb) 35, and auxiliary gas rate (arb) 15. Mass spectra were acquired in the m/z 150–2000 Da range. The LC-MS3 analysis was performed in data depending acquisition mode. Data were analyzed using Xcalibur software (Thermo Fisher Scientific Inc., Waltham, MA, USA, version 1.4 SR1).
All NMR spectra (1D 1H and 13C, COSY, DEPT, HMQC, HMBC and NOE), were recorded on a Bruker Avance-III-700 spectrometer (Bruker, Germany). Chemical shifts are reported in δ units with TMS (tetramethylsilane) as the internal standard.
3.2. Plant Material, Establishment of Cell Cultures, Plants and Bacterial Infection
Two species of Ornithogalum, O. dubium (#49_60) and O. thyrsoides (#95/36/1) were crossed to produce F1 interspecific hybrids, of which two lines were selected designated as #2_28 and #2_32 and cloned for further analyses [24]. Cell cultures and infection methods were executed according to previous works [6,13]. Briefly, 20 micropropagated plantlets of each of the F1 hybrids were inoculated by spraying 1 mL of fresh cultures of Pc 106 CFU mL−1 in a laminar flow hood. Bacterial cell cultures were washed twice and re-suspended in DDW before inoculation.
3.3. Extraction and Separation
Leaves of Ornithogalum were obtained from either greenhouse mature plants or plantlets from tissue cultures. The extraction of phenolics was executed 48 h post-inoculation of plantlets, and samples (20 g) were frozen in liquid nitrogen, ground and dried. Selective extraction of phenolic compounds from plant tissues was adjusted based on previous works [13,26,27]. Dry samples were suspended in an acidic methanol solution (100 mL/sample, MeOH80%/HCl0.1%), and kept stirred at 4 °C overnight. The supernatant was filtered through a double layer glass fiber (Whatman, 47 mm, 0.2 μm and Whatman GF/C 47 mm), acidified (pH = 2), and washed with hexane (x3, v/v). The aqueous layer was washed again with ethyl acetate (x3, Vol/Vol), to remove free phenolics (aglycones). The aqueous phase was evaporated to dryness under vacuum and reconstituted using 10 mL of methanol (HPLC grade), to a final concentration of 2 mg/mL. The samples were kept frozen (−20 °C) for future analyses.
3.4. Reversed Phase High-Performance Liquid Chromatography (RP-LC) Assay of Kaempferol
Fractions, isolated and collected by flash chromatography, were analyzed using an RP-LC system: TSP P4000 (Thermo Separation Products, Riviera Beach, FL, USA), consisting of an auto sampler (AS3000), pump (P3000), injector (100 µL), column oven (30 °C) and diode-array detector (UV6000). Prior to analysis, 100 μL of extracted sample in MeOH, was added to 100 μL of DDW. A reversed-phase (RP) C-18 column (Phenomenex, Luna, C18, 250 × 4.60 mm, 5 μm) was employed. Gradient elution was performed using water (A) and MeOH-ACN (1:1) (B). Initial conditions were 72% A, a linear gradient to 64% A from 2 min to 24 min, a second linear gradient to 100% B for 2 min, and held for 4 min at 100% B, at a flow rate of 1 mL/min. Absorption of flavonoids was monitored at 336 nm.
3.5. Solid Phase Extraction (SPE)
Pure compounds for spectral analyses were prepared using 50 mg/0.5 mL sep-pak™ C-18 columns (Strata, Phenomenex, Torrance, CA, USA), following RP-LC separations. Prior to use, columns were pre-conditioned with 5.0 mL of methanol, followed by 5.0 mL of double distilled water and finally 5.0 mL of 10% methanol in water. Before being loaded onto the column, aqueous methanol solution (10% volume) was added to each dried RP-LC fraction. Fractions (100 µL) were then eluted with MeOH (GC grade), UV absorbing fractions were dried under nitrogen stream.
3.6. Kaempferol O-tri-glycoside (1)
3.7. Kaempferol O-tri-glycoside (2)
3.8. Kaempferol O-tri-glycoside (3)
4. Conclusions
The induction of the synthesis of flavonoids in Ornithogalum hybrids following infection with the soft-rot pathogen Pc revealed three novel kaempferol O-tri-glycosides. The levels of these compounds correlated with increased resistance to Pc infection in the parent line O. thyrosoides (#36_1), and in the F1 hybrids #2_28 and #2_32. The results suggest that interspecific breeding may be a practical approach to fight bacterial soft rot in ornamental flower bulbs.
Author Contributions
A.G. performed the physiological experiments, K.S. performed the isolation of the compounds. H.E.G. did NMR and data analysis, Z.K. analyzed the MS results; I.Y., K.S., and Z.K. designed the experiments and contributed to the writing of the study.
Funding
We thank the chief Scientist for Agriculture grant number 256-0996 for financial support.
Conflicts of Interest
The authors declare no conflict of interest. The funders had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results.
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Sample Availability: Not available. |
Figure 1.
Characterization of Ornithogalum breeding lines: Disease development in Ornithogalum leaf discs (lines: #49_60, #36_1, #2_28 and #2_32) at 24 h after inoculation with 10 µL of Pectobacterium carotovorum suspension (108 CFU/mL). (A) Disease severity is expressed as total necrotic area. (B) Levels of polyphenolic compounds in methanolic extracts of Ornithogalum lines, expressed as catechin equivalents (mg/g fresh tissue). (C) RP-LC-UV chromatograms, at 336 nm, of extracts from all Ornithogalum lines: Clones #49_60 (black), #2_32 (pink), #2_28 (blue) and #36_1 (brown). (D) Flowering of the parental lines and F1 hybrids of the Ornithogalum that were used in the study.
Figure 1.
Characterization of Ornithogalum breeding lines: Disease development in Ornithogalum leaf discs (lines: #49_60, #36_1, #2_28 and #2_32) at 24 h after inoculation with 10 µL of Pectobacterium carotovorum suspension (108 CFU/mL). (A) Disease severity is expressed as total necrotic area. (B) Levels of polyphenolic compounds in methanolic extracts of Ornithogalum lines, expressed as catechin equivalents (mg/g fresh tissue). (C) RP-LC-UV chromatograms, at 336 nm, of extracts from all Ornithogalum lines: Clones #49_60 (black), #2_32 (pink), #2_28 (blue) and #36_1 (brown). (D) Flowering of the parental lines and F1 hybrids of the Ornithogalum that were used in the study.
Figure 2.
Mass spectra of Compound 1 in the negative mode, full mass spectrum (A), and MS3 (B).
Figure 3.
Kaempferol-O-tri-glycosides 1, 2 and 3.
Figure 4.
Selected HMBC correlations for compound 1.
Table 1.
13C- and 1H-NMR data (J in Hz) for the aglycone moiety of kaempferol glycosides 1, 2 and 3 in CD3OD.
Table 1.
13C- and 1H-NMR data (J in Hz) for the aglycone moiety of kaempferol glycosides 1, 2 and 3 in CD3OD.
| 1 | 2 | 3 | ||||
|---|---|---|---|---|---|---|
| 13C | 1H | 13C | 1H | 13C | 1H | |
| Kaempferol | ||||||
| 2 | 158.54 | — | 158.57 | — | 158.61 | — |
| 3 | 134.53 | — | 134.51 | — | 134.56 | — |
| 4 | 179.38 | — | 179.32 | — | 179.37 | — |
| 4a | 106.04 | — | 106.00 | — | 106.13 | — |
| 5 | 163.23 | — | 163.22 | — | 163.25 | — |
| 6 | 99.84 | 6.18 (d, 2) | 99.89 | 6.17 (d, 2) | 99.80 | 6.19 (d, 2) |
| 7 | 165.90 | — | 165.83 | — | 165.69 | — |
| 8 | 94.80 | 6.39 (d, 2) | 94.87 | 6.39 (d, 2) | 94.79 | 6.40 (d, 2) |
| 8a | 158.49 | — | 158.47 | — | 158.54 | — |
| 1′ | 123.10 | — | 123.08 | — | 123.09 | — |
| 2′, 6′ | 132.24 | 8.08 (AA’XX’) | 132.24 | 8.07 (AA’XX’) | 132.23 | 8.07 (AA’XX’) |
| 3′, 5′ | 116.20 | 6.90 (AA’XX’) | 116.16 | 6.89 (AA’XX’) | 116.22 | 6.91 (AA’XX’) |
| 4′ | 161.41 | — | 161.42 | — | 161.43 | — |
Table 2.
13C and 1H-NMR data (J in Hz) for the sugar units of kaempferol glycosides 1, 2 and 3 in CD3OD.
Table 2.
13C and 1H-NMR data (J in Hz) for the sugar units of kaempferol glycosides 1, 2 and 3 in CD3OD.
| 1 | 2 | 3 | ||||
|---|---|---|---|---|---|---|
| 13C | 1H | 13C | 1H | 13C | 1H | |
| Glucose | ||||||
| 1 | 100.26 | 5.65 (d, 7.5) | 100.34 | 5.64 (d, 7.5) | 100.25 | 5.64 (d, 7.5) |
| 2 | 80.51 | 3.61 (dd, 9.5,7.5) | 79.72 | 3.60 (dd, 9.5,7.5) | 80.41 | 3.62 (dd, 9.5,7.5) |
| 3 | 78.60 | 3.57 (dd, 9.5, 9) | 78.57 | 3.57 (dd, 9.5, 9) | 78.44 | 3.57 (dd, 9.5, 9) |
| 4 | 71.83 | 3.31 a | 71.84 | 3.29 a | 71.85 | 3.30 a |
| 5 | 77.58 | 3.41 (ddd, 9.5, 6, 2) | 77.58 | 3.40 (ddd, 9.5, 6, 2) | 77.58 | 3.41 (ddd, 9.5, 6, 2) |
| 6 | 69.46 | 3.57 (dd, 12, 6) | 69.47 | 3.56 (dd, 12, 6) | 69.47 | 3.58 (dd, 12, 6) |
| 3.90 (dd, 12, 2) | 3.90 (dd, 12, 2) | 3.90 (dd, 12, 2) | ||||
| Xylose | ||||||
| 1 | 105.14 | 4.07 (d, 7.5) | 105.14 | 4.06 (d, 7.5) | 105.15 | 4.07 (d, 7.5) |
| 2 | 74.76 | 2.99 (dd, 9, 7.5) | 74.75 | 2.99 (dd, 9, 7.5) | 74.75 | 3.00 (dd, 9, 7.5) |
| 3 | 77.42 | 3.08 (t, 9) | 77.42 | 3.09 (t, 9) | 77.41 | 3.09 (t, 9) |
| 4 | 71.02 | 3.34 a | 71.02 | 3.33 a | 71.04 | 3.34 a |
| 5 | 66.54 | 2.88 (dd, 11.5, 10) | 66.54 | 2.87 (dd, 11.5, 10) | 66.53 | 2.88 (dd, 11.5, 10) |
| 3.64 (dd, 11.5, 5) | 3.64 (dd, 11.5, 5) | 3.65 (dd, 11.5, 5) | ||||
| Rhamnose | ||||||
| 1 | 102.70 | 5.20 (d, 2) | 100.25 | 5.21 (d, 2) | 100.07 | 5.20 (d, 2) |
| 2 | 69.99 | 4.18 (dd, 3.5, 2) | 74.22 | 5.23 (dd, 3.5, 2) | 71.44 b | 5.42 (dd, 3.5, 2) |
| 3 | 75.98 | 5.05 (dd, 10, 3.5) | 70.52 | 3.97 (dd, 10, 3.5) | 73.50 | 5.18 (dd, 10, 3.5) |
| 4 | 71.30 | 3.55 (t, 10) | 74.27 | 3.30 (t, 10) | 71.51 b | 3.46 (t, 10) |
| 5 | 70.10 | 4.20 (dd, 10, 6) | 70.10 | 4.10 (dd, 10, 6) | 70.02 | 4.24 (dd, 10, 6) |
| 6 | 17.58 | 1.00 (d, 6, 3H) | 17.61 | 1.00 (d, 6, 3H) | 17.55 | 1.02 (d, 6, 3H) |
| 2-Ac | — | — | 20.98 | 2.09 (s, 3H) | 20.94 | 2.01 (s, 3H) |
| — | — | 172.31 | — | 171.79 | — | |
| 3-Ac | 21.16 | 2.11 (s, 3H) | — | — | 20.75 | 2.09 (s, 3H) |
| 172.82 | — | — | — | 172.41 | — | |
a Signal under solvent residual peak. b Chemical shifts may be interchanged.
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Yedidia, I.; Schultz, K.; Golan, A.; Gottlieb, H.E.; Kerem, Z. Structural Elucidation of Three Novel Kaempferol O-tri-Glycosides that Are Involved in the Defense Response of Hybrid Ornithogalum to Pectobacterium carotovorum. Molecules 2019, 24, 2910. https://doi.org/10.3390/molecules24162910
AMA Style
Yedidia I, Schultz K, Golan A, Gottlieb HE, Kerem Z. Structural Elucidation of Three Novel Kaempferol O-tri-Glycosides that Are Involved in the Defense Response of Hybrid Ornithogalum to Pectobacterium carotovorum. Molecules. 2019; 24(16):2910. https://doi.org/10.3390/molecules24162910
Chicago/Turabian StyleYedidia, Iris, Keren Schultz, Avner Golan, Hugo E. Gottlieb, and Zohar Kerem. 2019. "Structural Elucidation of Three Novel Kaempferol O-tri-Glycosides that Are Involved in the Defense Response of Hybrid Ornithogalum to Pectobacterium carotovorum" Molecules 24, no. 16: 2910. https://doi.org/10.3390/molecules24162910
