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Abstract

Erycina pusilla is a miniature orchid species native to tropical America. The unique characteristics, e.g., a short juvenile stage and its miniature size, make E. pusilla a perfect material for producing the ornamental flask product and for the study of flowering physiology and in vitro breeding. In this chapter, we demonstrate the culture steps of in vitro flowering of E. pusilla. Using method detailed, it is possible to produce blooming E. pusilla plants within 1 year from in vitro seed germination and producing ornamental flasks for commercial sale. This culture protocol can also be used for in vitro breeding to select additional desirable traits for ornamental flask production.

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Reference

  1. Murthy KSR, Kondamudi R, Chalapathi RPV, Pullaiah T (2012) In vitro flowering-a review. J Agric Technol 8:1517–1536

    Google Scholar 

  2. Chang C, Chang WC (2003) Cytokinins promotion of flowering in Cymbidium ensifolium var. misericors in vitro. Plant Growth Regul 39:217–221

    Article  CAS  Google Scholar 

  3. Vaz APA, Càssia RD, Figueiredo-Ribeiro L, Kerbauy GB (2004) Photoperiod and temperature effects on in vitro growth and flowering of P. pusilla, an epiphytic orchid. Plant Physiol Biochem 42:411–415

    Article  CAS  Google Scholar 

  4. Teixeira da Silva JA, Kerbauy GB, Zeng S, Chen Z, Duan J (2014) In vitro flowering of orchids. Crit Rev Biotechnol 34:56–76

    Article  Google Scholar 

  5. Lopez RG, Runkle ES (2005) Environmental physiology of growth and flowering of orchids. HortSci 40:1969–1973

    Google Scholar 

  6. Hew CS, Yong JWH (1997) Physiology of tropical orchids in relation to the industry. World Scientific Publishing Co. Pte Ltd, Singapore

    Book  Google Scholar 

  7. Williams NH, Chase MW, Fulcher T, Whitten WM (2001) Molecular systematics of the Oncidiinae based on evidence from four DNA sequence regions: expanded circumscriptions of Cyrtochilum, Erycina, Otoglossum, and Trichocentrum and a new genus (Orchidaceae). Lindleyana 16:113–139

    Google Scholar 

  8. Dodson CH, Dressler RL (1972) Two undescribed genera in the Orchidaceae - Oncidiinae. Phytologia 24:285–292

    Google Scholar 

  9. Dodson CH (1957) Oncidium pusillum and its allies. Amer Orchid Soc Bull 26:170–172

    Google Scholar 

  10. Livingston R b (1962) Oncidium pusillum- blooming in flask! Amer Orchid Soc Bulletin 31:1007

    Google Scholar 

  11. Vaz APA, Kerbauy GP (2000) Effects of mineral nutrients on in vitro growth and flower formation of Psygmorchis pusilla (Orchidaceae). Acta Hort 520:149–156

    Article  Google Scholar 

  12. Vaz APA, Kerbauy GB (2008) In vitro flowering studies in Psygmorchis pusilla. Glo Sci 42:427–432

    Google Scholar 

  13. Baker ML, Baker CO (2006) Orchid species culture: oncidium/odontoglossum alliance. Timber Press, Portland, OR, p 992

    Google Scholar 

  14. Chiu YT, Lin CS, Chang C (2011) In vitro fruiting and seed production in Erycina papilla (L.) N.H. Williams & M.W. Chase. Prop Ornamental Plants 11:131–136

    Google Scholar 

  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

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Correspondence to Chen Chang .

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Chiu, YT., Chang, C. (2018). In Vitro Flowering and Breeding of Erycina pusilla . In: Lee, YI., Yeung, ET. (eds) Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7771-0_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7771-0_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7770-3

  • Online ISBN: 978-1-4939-7771-0

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